New Shunosaurus (Dinosauria: Sauropoda) material from the middle Jurassic lower Shaximiao Formation of Yunyang, Chongqing, China

AUTHORS

Qingyu Ma, Hui Dai,Chao Tan, Ning Li, Ping Wang, Xinxin Ren, Li Meng, Qi Zhao, Guangbiao Wei & Xing Xu

ABSTRACT

Shunosaurus is a small eusauropod from China. It is characterised by solid cervical and dorsal vertebral centra without complicated pneumatic structures, platycoelous or amphicoelous middle and posterior vertebrae and a lack of pubic foramen in adult individuals. Although many Shunosaurus individuals have been discovered, the ontogenetic characteristics of its long bones and bone tissues are not very clear and the existing description of the postcranial skeleton is relatively rudimentary. The new well-preserved and the smallest Shunosaurus specimen discovered in Yunyang, Chongqing, China, provides good material for solving these problems. The radial distal breadth is more than twice the minimum midshaft breadth, and the fibular distal end is twice as wide as the midshaft, while these ratios are all smaller in adult specimens. The lateral trochanter is undeveloped. This individual does show a pubic foramen. The degree of vascularisation of the bone tissue in the juvenile bone tissue is higher for adults. There are no arrested growth lines and peripheral rest lines in the compact bone, indicating that body size still increases slowly after maturity. The discovery of this new material expands the distribution range of Shunosaurus in China.

RhoA/Rock activation represents a new mechanism for inactivating Wnt/β-catenin signaling in the aging-associated bone loss

AUTHORS

Wei Shi, Chengyun Xu, Ying Gong, Jirong Wang, Qianlei Ren, Ziyi Yan, Liu Mei, Chao Tang, Xing Ji, Xinhua Hu, Meiyu Qv, Musaddique Hussain, Ling-Hui Zeng & Ximei Wu

ABSTRACT

The Wnt/β-catenin signaling pathway appears to be particularly important for bone homeostasis, whereas nuclear accumulation of β-catenin requires the activation of Rac1, a member of the Rho small GTPase family. The aim of the present study was to investigate the role of RhoA/Rho kinase (Rock)-mediated Wnt/β-catenin signaling in the regulation of aging-associated bone loss. We find that Lrp5/6-dependent and Lrp5/6-independent RhoA/Rock activation by Wnt3a activates Jak1/2 to directly phosphorylate Gsk3β at Tyr216, resulting in Gsk3β activation and subsequent β-catenin destabilization. In line with these molecular events, RhoA loss- or gain-of-function in mouse embryonic limb bud ectoderms interacts genetically with Dkk1 gain-of-function to rescue the severe limb truncation phenotypes or to phenocopy the deletion of β-catenin, respectively. Likewise, RhoA loss-of-function in pre-osteoblasts robustly increases bone formation while gain-of-function decreases it. Importantly, high RhoA/Rock activity closely correlates with Jak and Gsk3β activities but inversely correlates with β-catenin signaling activity in bone marrow mesenchymal stromal cells from elderly male humans and mice, whereas systemic inhibition of Rock therefore activates the β-catenin signaling to antagonize aging-associated bone loss. Taken together, these results identify RhoA/Rock-dependent Gsk3β activation and subsequent β-catenin destabilization as a hitherto uncharacterized mechanism controlling limb outgrowth and bone homeostasis.